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Synthesis of RNA–Digoxigenin Probe
This protocol describes the synthesis of an RNA probe labeled with digoxigenin (DIG) for use in whole mount in situ hybridization (WISH). The...
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Spectropolarimeter (Jasco-1500) Operation Protocol
The Jasco-1500 spectropolarimeter is a versatile instrument used for circular dichroism (CD) spectroscopy to analyze the secondary structure of...
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The Production and Characterization of Synthetic Glycoconjugate Vaccines
Chemical conjugation of polysaccharides to protein carriers has become essential in carbohydrate vaccine development to enhance the poor...
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Retrotranscription via STEM LOOP
This protocol outlines the retrotranscription (reverse transcription) of miRNAs for RT-qPCR using the StemLoop method.
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Thermal Difference Spectra (TDS)
Thermal difference spectra (TDS) is a technique used to study the thermal stability and conformational changes of biomolecules by measuring the...
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Viral Vector-Based Cancer Vaccines
Viral vectors have been frequently used as vaccine-delivery vehicles. Both DNA and RNA viruses have been employed for vaccine development. Viral...
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Protocol for Isolation of Lung Fibroblasts from Mouse Tissue
This protocol describes the process for isolating lung fibroblasts from mouse tissue, which are commonly used in studies of lung biology, fibrosis,...
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Dendritic Cell Cancer Vaccines: A Focused Review
Dendritic cells (DCs) are the most potent professional antigen-presenting cells to activate both CD4+ and CD8+ T lymphocytes. When immature, they...
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Dendritic Cell Cancer Vaccines: Clinical Production for Cancer Immunotherapy
Dendritic cell (DC) cancer vaccines are used to circumvent the problem that DCs in patients with cancer usually do not mature properly in the cancer...
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Making Universal Vaccines and Antibodies Through Glycoengineering
Biological glycosylation is a process used by nature to modulate the structure and function of biomolecules, particularly the glycoproteins on the...
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The Tumor Immune Environment: Advances in the Cancer Immunotherapy Era
For over the last hundred years, the scientific community has demonstrated much interest in the roles of the immune system in regulating tumor...
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Mouse Model of Brain Tumor Resection
Glioblastoma is one of the most common and aggressive primary brain tumors, displaying poor survival rates and little success in clinical trials....
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3D Walking Nanomachine for Monitoring of miRNA in Living Cells
Three-dimensional (3D) DNA walking nanomachines have been widely applied in molecular diagnostics and cancer therapy. Specially, DNA dendrimer with...
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Generation of Biologically Contained Marburg Virus
Wild-type Marburg virus (MARV) can only be handled in biosafety level 4 facilities. By removing an essential gene from the virus genome, deficient...
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Evaluation of Marburg Virus Medical Countermeasures in Guinea Pigs
Various animal models have been established to gain a better understanding of the pathogenesis of Marburg virus (MARV) and Ravn virus (RAVV), and to...
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Marburg Virus Medical Countermeasures
Among the Filoviridae, Marburg virus (MARV) is a biological threat for which no licensed vaccines or therapeutics are currently available. In...
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Evaluating Countermeasures Against Marburg Virus Using a Mouse Model
Animal models are key tools for understanding Marburg virus (MARV) pathogenesis and evaluating novel countermeasures. RodentsRodents, in particular,...
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Visualizing the Internalization of Marburg Viruslike Particles into Living Cells
Viral entry into cells is a pivotal stage of the infection process and, therefore, a prime target for the development of antiviral therapeutics....
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NBS-Predict: An Easy-to-Use Toolbox for Connectome-Based Machine Learning
NBS-Predict is a prediction-based extension of the Network-based Statistic (NBS) approach, which aims to alleviate the curse of dimensionality, lack...
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Actionable Event Annotation and Analysis in fMRI: A Practical Guide to Event Handling
Many common analysis methods for task-based functional MRI rely on detailed information about experiment design and events. Event recording and...